Tuesday Poster Sessions, October 30, 2006

Clinical Trials and Therapeutic Developments

 

 

1123. HCV RNA Negativity After 12 Weeks of Therapy is the Best Predictor of Sustained Viral Response (SVR) in the Re-Treatment of Previous Interferon-α/Ribavirin Non-Responders (NR): Results from the EPIC3 Program. 

T. Poynard; E. Schiff; R. Terg; R. Moreno Otero; S. Flamm; W. Schmidt; T. Berg; F. Goncales; J. Heathcote; M. Diago; T. McGarrity; A. Maieron; J. Reichen; H. Tanno; C. Brandao; J. McHutchison; M. Silva; P. Bedossa; W. Deng; P. Mukhopadhyay; L. Griffel; M. Burroughs; C. Brass; J. K. Albrecht.

 

Introduction:

The EPIC3 program includes a large, prospective, controlled trial designed to assess the safety and efficacy of re-treatment with peginterferon α-2b (PEG) and ribavirin (RBV) of subjects who have failed previous treatment with any α-interferon plus ribavirin (I/R), including those that had failed PEG/RBV or peginterferon alfa-2a/RBV. We have previously reported a surprisingly high SVR in these patients, especially those with an early viral response.

 

Aim:

To define early viral response at week 12 as a predictor of SVR in these patients.

 

Methods:

HCV NRs or those that had relapsed after previous treatment with I/R who have significant fibrosis (Metavir F2-F4) received PEG-Intron 1.5 microgram/kg subcutaneously once weekly plus Rebetol 800-1400 mg/day for up to 48 weeks. All patients had pre-treatment biopsies scored by a single reviewer using METAVIR criteria. Plasma HCV-RNA was determined at weeks 12, 24 and 48 of therapy and FU 12 and 24 using a quantitative Taq-Man assay (SPRI; sensitivity 29 IU/mL). Genotype was determined by sequencing PCR product.

 

Results:

Of the first 1354 patients treated in the combination therapy trial, 23% achieved SVR. Of those who attained > 2 log decrease in viral load at week 12, 37% achieved SVR: 56% of those who were HCV-RNA (-), but only 6% of those who attained a 2 log decrease in HCV-RNA but remained HCV-RNA positive. Of this latter group, 17% of subjects with very low viral load at TW12 (<100 IU) achieved SVR compared to 5% of those with residual viral load of >100-250IU, and 0 in those with HCV-RNA >750.

 

Conclusions:

SVR is strongly correlated with a negative HCV-RNA or HCV-RNA near the lower limit of detection at week 12, but is extremely low in those with HCV-RNA>100 IU/ml. These data suggest that undetectable viral load at week 12 (<29 IU/ml) best defines a robust EVR that predicts SVR for previous I/R treatment failures re-treated with PEG-Intron plus WBD ribavirin.

 

Rate of SVR based on viral load at TW12

HCV-RNA at TW 12 (I.U./mL)

SVR % (n/total)

>750

0 (0/582)

>500-750

4.8 (1/21)

>250-500

5.1 (2/39)

>100-250

5.1 (2/39)

29-100

16.9 (13/77)

Undetectable

56.1 (281/501)

 


1124. PREDICTORS OF RAPID VIROLOGIC RESPONSE (RVR) IN HCV GENOTYPE 1 CHRONIC INFECTED PTS: RESULTS OF A RANDOMIZED CONTROLLED TRIAL ON INDIVIDUALIZED TREATMENT. 

A. Mangia; N. Minerva; V. Carretta; D. Bacca; G. L. Ricci; F. Vinelli; R. Cozzolongo; O. Vuturo; S. Gaetano; F. Spirito; L. Mottola; D. Petruzzellis; A. Andriulli.

 

Objectives:

To prospectively compare the efficacy of PEG interferons (IFNs) alpha 2a or 2b and Ribavirin (RBV) when given for the standard 48 wks or as individualized treatment, for 24, 48 or 72 wks in accordance with HCVRNA negative by PCR at different time points in naïve pts with HCV genotype 1. To identify predictive factors of both 1) HCVRNA negative test at wk 4 (RVR), and 2) sustained virological response (SVR).

 

Methods:

A total of 645 naïve pts were enrolled and randomly assigned at 2:1 ratio to a standard 48-wk regimen of PEGIFNs (alternatively alpha 2b, 1.5 mcg/wk or alpha 2a, 180 mcg/wk) plus ribavirin (RBV) 1000-1200 mg/daily on the basis of body weight (n= 215, Group B) or to an individualized treatment based on HCVRNA evaluation at different time points, with the same PEGIFNs and RBV doses (n= 430, Group A). When HCV was firstly not detectable (< 50 UI /ml) at wks 4, 8 or 12, pts in individualized treatment group were treated for 24, 48, 72 wks, respectively. Pts RNA positive at wk 12 were considered non responders. Primary end point was SVR (HCVRNA <50 IU/ml ) 24 wks after the end of treatment.

 

Results: 

Both groups were well matched for baseline characteristics. By wk 8, 54% out of 645 patients were HCVRNA-ve. Overall SVR was 46.2% with the individualized and 44.6% with the standard treatment (p= 0.55). RVR was achieved in 28% of pts (n= 184) in both groups. Of these rapid responders 76.5% in group A and in 84.1% in Group B obtained SVR (p=0.25). RVR was achieved in 29.7% of pts treated with alpha 2b and 27.2% of those treated with alpha 2a. Relapse rate was 18% among pts treated for 24 wks, but 10.5% overall in Group A, and 9.5% in Group B. No differences were found on the rate of relapse between the two IFNs (10.1 vs 10.5%). By univariate analysis, factors associated with RVR were age ≤45 yrs (p=0.01), HCVRNA levels lower than 600.000 IU/ml (p= 0.01) and fibrosis score < 3 (0.008). Factors associated with SVR in the table. The independent predictor of 1)RVR was fibrosis score <3, p=0.024; OR 1.27, 95% CI 1.03-1.57, 2)SVR was RVR, p=0.0001, OR 8.6, 95% CI 5.3-13.9.

 

Conclusions: 

In HCV1 pts, once RVR is obtained, a 24 wks course of treatment is effective as a 48 wks course. Pts with advanced liver damage are less likely to achieve RVR and to be treated for only 24 wks. RVR is the best predictor of SVR. The highest likelihood of SVR is achieved by wk 8.

 

 

NR (N=335)

SVR(N=273)

P value

Age<45 yrs

87

104

0.002

Fibrosis < 3

169

180

0.0001

RVR

38

143

0.0001

Drop out

58

0

0.0001

 


1125. Peripheral blood gene expression profiles predict sustained virologic response following peginterferon and ribavirin therapy. 

C. Huang; C. William; H. Chen; C. D. Howell.

 

Introduction:

There is a need for biomarkers that when measured either before or during the first month of treatment can accurately predict the outcomes of HCV therapy. Goal: We conducted a study of global gene expression in the peripheral blood mononuclear cells (PBMC) of HCV genotype 1 patients during the first 4 weeks of peginterferon (PEGIFN) plus ribavirin treatment and defined a minimal set of genes for predicting a SVR and no SVR.

 

Methods:

Treatment-naïve, HCV genotype 1 patients (n = 24) were treated with PEGIFN alfa-2a (180 mcg/wk) plus ribavirin (1000-1200 mg per day) for 48 weeks. PBMC were isolated at baseline (day - 0) before treatment and at day-28 of therapy. PBMC RNA was amplified one time and hybridized to the Affymetrix GeneChip HG-U133 plus 2.0 array representing 39,000 human genes. Significance Analysis of Microarray (SAM) software was used to identify genes expressed differentially between day-0 and day-28 and between SVR (undetected serum HCV RNA > 24 weeks after treatment) and non-SVR patients. Genes were classified by biological process and ranked using DAVID 2.0. Prediction Analysis of Microarray (PAM) software was used to select sets of differentially expressed genes to predict SVR and to determine the misclassification rate.

 

Results:

Compared to day 0 (pretreatment baseline), the expression of 309 genes was increased significantly and the expression of 668 genes was decreased at day 28 with a false discovery rate of <1.0 %. A large number of interferon-stimulated genes were increased with response to pest, pathogen or parasite (p < 0.0001), response to virus (p < 0.0001), host defense response (p < 0.0001), inflammatory response (p < 0.001), protein kinase cascade (p < 0.001), chemotaxis (p < 0.05), protein modification (p < 0.05), and regulation of NF-kappa B (p < 0.05) being the most significant biological processes. DNA dependent RNA transcription, RNA processing, and protein biosynthesis, modification, and transport (p < 0.001 for each category) were the main processes associated with the decreased genes. The microarray results were confirmed by realtime RT-PCR for 10 select genes. PAM identified differentially expressed gene subsets from 13 to 124 in number that correctly predicted SVR and no-SVR among with a misclassification (i.e. error) rate of <10%.

 

Conclusions:

PEGIFN alfa-2a and ribavirin treatment is associated with significant alterations in PBMC gene expression during the first 28 days. A change in expression of a limited set of genes at day-28 was able to predict SVR with greater than 90% accuracy.

 


1126. Hepatitis C virus directly causes insulin resistance independent of the visceral adipose tissue area in non-obese and non-diabetic Japanese patients. 

M. Yoneda; K. Fujita; T. Fujisawa; H. Chiba; H. Mawatari; H. Takahashi; A. Goto; H. Kirikoshi; K. Yonemitsu; S. Saito; A. Nakajima.

 

Introduction:  

Insulin Resistance (IR) is associated with obesity, type 2 DM and in particular, the visceral adipose tissue area. However, none of the epidemiological studies conducted until now have analyzed the relationship between HCV infection and the risk of IR independent of the influence of obesity, type 2 DM and heavy alcohol consumption; therefore, the precise relationship between HCV and IR remains unclear. Elucidation of the relationship between HCV and IR is of great clinical relevance, because IR promotes liver fibrosis. In this study, we tested the hypothesis that HCV infection by itself may promote IR, independent of the influence of the visceral adipose tissue area, in non-obese, non-diabetic and non-alcoholic patients with chronic HCV infection.

 

Material and Methods:

We prospectively evaluated 47 patients with chronic HCV infection who underwent liver biopsy. Patients with obesity (BMI >25), type 2 DM, and a history of alcohol consumption were excluded from this study. IR was estimated by calculation of the modified homeostasis model of insulin resistance (HOMA-IR) index. The abdominal fat distribution in the subjects was determined by computed tomography (CT).

 

Results:

Fasting blood glucose levels were within normal range in all the patients. The results of univariate analysis revealed a significant correlation between the quantity of HCV RNA and the HOMA-IR. While a significant correlation between the visceral adipose tissue area and the HOMA-IR was observed in the 97 control non-diabetic, non-HCV-infected patients (r= 0.398, p<0.0001. However, to our surprise, no such significant correlation between the visceral adipose tissue area and the HOMA-IR (p= 0.04465) was observed in the patients with HCV infection. Multiple regression analysis with adjustment for age, gender and visceral adipose tissue area revealed a significant correlation between the HCV-RNA titer and the HOMA-IR (p= 0.0446).

 

Conclusion:

Hepatitis C virus directly causes IR in a dose-dependent manner independently of the visceral adipose tissue area before the onset of diabetes. This is the first report to demonstrate the direct involvement of HCV and IR in patients with chronic HCV infection.

 


1127. Analysis of HCV NS5B Genetic Variants Following Monotherapy with HCV-796, a Non-Nucleoside Polymerase Inhibitor, in Treatment-Naïve HCV-Infected Patients. 

S. Villano; A. Howe; D. Raible; D. Harper; J. Speth; G. Bichier.

 

Background:

HCV-796, an oral non-nucleoside inhibitor of hepatitis C virus (HCV) RNA-dependent RNA polymerase, has demonstrated potent antiviral activity in vitro and in vivo. In a clinical dose-ranging study, HCV-796 was administered twice daily for 14 days and demonstrated dose-related antiviral activity across multiple HCV genotypes, with maximal effects observed at approximately day 4 of treatment. Mean reductions of HCV RNA at day 4 in the highest dose groups were 1.4-1.5 log10 below baseline. In all dose groups, the predominant virologic response pattern demonstrated an initial decline of plasma HCV RNA levels followed by an increase of viral RNA levels that were not explained by changes in plasma concentrations of HCV-796.

 

Methods:

In a randomized, double-blind, placebo-controlled study, 102 treatment-naïve adult patients with HCV (72% genotype 1) received 50, 100, 250, 500, 1000, or 1500 mg oral doses of HCV-796 or placebo given as monotherapy twice daily (q12h) for 14 days. Samples from baseline, during treatment, and at the end of treatment were selected for analysis from patients with various virologic response patterns (no response, initial decline in HCV RNA levels followed by an increase, and continuous decline). HCV RNA from plasma was isolated, and full length NS5B was amplified using RT-PCR and then sequenced.

 

Summary/Conclusion:

Sequence data of the NS5B gene are available for 58 patients with HCV genotype 1a.

 

·        HCV-796 demonstrated initial rapid antiviral activity in treatment-naïve patients with HCV infection.

·        Subsequent increases of plasma HCV RNA levels during HCV-796 monotherapy appear to be associated with selection of viral variants with reduced susceptibility to the inhibitor.

·        The major variant expresses a C316Y substitution in NS5B, and remains susceptible to interferon and ribavirin in vitro.

·        C3164/f/s replicons results in 10- to 166 fold reduced susceptibility to inhibition by interferon, ribavirin, and other HCV inhibitors

·        Several baseline and on-therapy mutations were identified in patients who did not achieve continued virologic response

·        Analyses of additional subjects and timepoints are in progress.

·        Validity of these mutation need to be confirmed in phase II studies

 


1128. Extended Treatment of 72 versus 48 Weeks for Chronic Hepatitis C Patients with Genotype 1 and High Viral Load using Daily Consensus Interferon and Ribavirin. 

P. Kaiser; H. Holger; L. Bissinger; L. Bettina; B. Sauter; M. Gregor.

 

Treatment of naive, genotype 1, high viral load chronic Hepatitis C patients with pegylated interferon and ribavirin (RBV) results in sustained viral response rates of about 30 –35 %. Recently improved response rates have been suggested in treatment trials using an extended treatment duration of 72 weeks. Furthermore, treatment with an bio-optimized interferon, Consensus interferon (CIFN), has shown higher response rates in difficult-to-treat patients such as nonresponders and relapsers.

 

Methods:

The efficacy of CIFN daily dosing + RBV for 48 versus 72 weeks in genotype 1, high viral load patients was evaluated. 120 patients have been included, average weight of patients was 79 kg. Patients were either treated with CIFN at 9 ug QD for 48 or 72 weeks both in combination with weight-based RBV.  All patients had HCV RNA >600 KIU.

 

Results:

Data show that after the initial 12 weeks a primary response with a 2-log drop of HCV-RNA (EVR) was observed in 89% and 92% of patients in the CIFN QD 48 week and 72 week treatment group. The sustained viral response rates (SVR) were 51 and 68 %, respectively (p<0.05), indicating a significantly higher relapse rate in patients treated with CIFN for 48 weeks compared to 72 weeks. No growth factors were used in this study. Three patients experienced grade III thrombocytopenias, while no grade IV neutropenias or thrombocytopenias were observed. The overall tolerability of the CIFN QD regimen was comparable to PEG IFN standard combination therapy, while the 72 week regimen lead to a higher rate of injection site reactions and a slightly higher drop out rate of 7% versus 4% (p=n.s.).

 

Conclusions:

Extended CIFN daily dosing combination therapy for 72 weeks shows promising response rates in difficult-to-treat patients with genotype 1 and high viral load when compared to a standard treatment period of 48 weeks. Although a significant proportion of patients also experienced a relapse after cessation of a 72 week treatment period, the overall sustained response rates are nevertheless promising showing a SVR in 68% of patients. It is concluded that extended treatment with CIFN in combination with RBV may be an effective treatment modality for this difficult-to-treat patient group.  In addition, a larger prospective study is underway to confirm these findings.

 


1129. Treatment of Hepatitis C Patients with Child A and B Cirrhosis with Consensus Interferon and Ribavirin in a Low Ascending Dosing Regimen Leads to Significant Viral Elimination Rates. 

P. Kaiser; L. Bissinger; H. Hass; B. Lutze; B. Sauter; M. Gregor.

 

Objective:

Antiviral treatment response in patients with chronic hepatitis C and liver cirrhosis is considerably lower than in non-cirrhotic patients and therapy is complicated by high dropout rates, less tolerablity of side effects and high rates of hematological complications.

 

Pegylated interferons have shown higher response rates than standard interferons, however, also higher dose-reduction and drop-out rates due to lower tolerability, especially regarding thrombocytopenia, thus resulting in an overall only minor benefit. Consensus interferon (CIFN) is an interferon with a relatively low half-life, but stronger antiviral potency as shown by high efficacy in nonresponders.

 

Methods:

The efficacy of CIFN together with ribavirin (RBV) was evaluated in 114 patients with chronic hepatitis C and cirrhosis Child A and B. All patients had histologically proven cirrhosis, elevated ALT values and were viremic, with 79% having genotype 1. Child A patients were treated with CIFN 9 ug TIW for 4 weeks, followed by 9 ug QD for another 4 weeks. Continuing treatment consisted of CIFN 9 ug QD with RBV with a stepwise increase from 400 mg by 200 mg increments at 4 week intervals for a total of another 52 weeks. For Child B patients the two lead-in phases with CIFN monotherapy were extended to 6 weeks each, and the starting dose of RBV was 200mg with an otherwise identical therapy as with Child A patients. Based on tolerability the dosing of RBV was increased to a weight-based dosing for all patients.

 

Results:

At 60 weeks therapy an undetectable HCV-RNA was observed in 76% and 47% of Child A and B patients, respectively, with drop out rates of 13% and 27%. Sustained response rates showed a 64% and 29% response for Child A and B, respectively. Due to side effects CIFN had to be dose reduced in 11% and 31%, mainly due to low platelet counts. As growth factors erythropoetin as well as G-CSF was used. Three patients experienced grade III and one patients a grade IV thrombocytopenia. Overall tolerability of the CIFN QD regimen was comparable to a standard therapy with pegylated IFN and RBV, while CIFN even as QD treatment resulted in a lower rate of thrombocytopenias.

 

Conclusions:

CIFN as a low ascending and finally daily dosing regimen with subsequent escalating RBV shows significant response rates in Child A and B cirrhotic patients. Therapy is also safe, however, a significant portion of patients was unable to even tolerate lower doses of CIFN or RBV. These data suggest that for a subgroup of cirrhotic patients even in stage Child B a combination therapy of CIFN and RBV may lead to viral eradication.

 


1130. The cyclophilin inhibitor DEBIO-025 has a potent dual anti-HIV and anti-HCV activity in treatment-naïve HIV/HCV co-infected subjects. 

R. Flisiak; A. Horban; J. Kierkus; J. Stanczak; I. Cielnak; G. P. Stanczak; A. Wiercinska-Drapalo; E. Siwak; J. Higersberger; C. Aeschlimann; P. Grosgurin; V. Nicolas; J. Dumont; H. Porchet; R. Crabbé; P. Scalfaro.

 

Background:

In Poland almost 50% of HIV-1 infected patients are HCV co-infected. Available therapies do not include agents with a dual antiviral activity. DEBIO-025, a cyclophilin inhibitor, showed strong anti-viral activity in in vitro and in vivo studies, both against HIV-1 and HCV. This phase I study aimed to determine the anti-viral effect, the pharmacokinetic profile and the safety of an oral therapy with DEBIO-025.

 

Methods:

Two centers, double blind, placebo controlled, oral dosing, single arm, phase I study design. Treatment-naïve HIV-1 mono- or HIV/HCV co-infected subjects received 1200 mg of DEBIO-025 or placebo twice daily during 15 days (unbalanced randomization). DEBIO-025 blood and plasma levels were assessed on days 1 and 15. Viral loads were determined with the Abbott Realtime m2000 PCR system (HCV limit of quantification LOQ=10 IU/mL, HIV-1 LOQ= 25 copies/mL) daily from day -1 to 2, at days 4, 8, 10, 15 of treatment and at 1, 2 and 3 weeks after the last dose.

 

Results:

23 Caucasian subjects (19 DEBIO-025, 4 placebo) completed the study. DEBIO-025 was rapidly absorbed with peak plasma levels reached after 2 hours and a terminal half life of 100 hours. The mean maximal decrease in HIV-1 load was 1.0 log10 (±0.1 SE, p<0.001). A pronounced effect on HCV load was found with a mean maximal decrease of 3.6 log10 (±0.4 SE, p<0.001). All subjects, except one, showed a HCV reduction of >2 log10 with differences depending on genotype (GT; Tab). Three subjects had undetectable HCV plasma levels. Hyperbilirubinaemia was observed in 10 subjects and led to premature treatment discontinuation in 4. No increase of ALT/AST or γ-GT was observed. A platelet decrease was reported on 3 occasions, but was not associated with signs of bleeding. Abnormal bilirubin and platelets returned to baseline after end of treatment.

 

Safety:

·        Hyperbilirubinaemia in 10 subjects

·        A total of 4 patients discontinued treatment prematurely for this reason

·        Bilirubin levels returned to normal after cessation of treatment

·        This was not observed in doses up to 1200mg od for 10 days

·        Decreased plates in 2 patients but was not associated with signs of increased bleeding and patients’ platelets returned to normal.

 

Conclusions:

DEBIO-025 administered for 15 days showed a dual anti-viral effect in HIV-1 and HCV co-infected treatment naïve subjects and appears to be a very promising compound with a new and unique mode of action. Despite good clinical tolerance, the observation of hyperbilirubinaemia and platelet decrease requires further investigation.


1131. High-dose Pravastatin (Prava) 80mg/day is Associated with a Reduction in ALT values among Hypercholesterolemic Patients with Chronic Hepatitis C (CHCV) Infection: Analysis of a Prospective, Randomized , Double-blind, Placeo-controlled Trial. 

J. H. Lewis; S. F. Zweig; R. Belder.

 

Introduction:

The use of HMG-CoA reductase inhibitors (statins) is generally not recommended in pts with chronic liver disease (CLD), although retrospective analyses of statins indicate their safe use in patients’ with elevated ALT (due mostly to NAFLD) and a recent prospective, randomized ,placebo-controlled trial demonstrated the safety and efficacy of pravastatin in hypercholesterolemic subjects with various CLD [Gastroenterology 2006;130(4 suppl 2):A65 abstract #446].

 

Aim:  

We performed a subgroup analysis of subjects with CHCV from our 36-week study of Prava 80mg/day conducted in 320 hypercholesterolemic pts with various well-compensated CLDs (about 25% with CHCV and two-thirds having NAFLD). No statisically signifcant differences were observed between Prava or placebo recipients at any time point in terms of the primary safety endpoint of a doubling of ALT from either a normal or elevated baseline for the cohort as a whole. We did, however, observe a numerically lower number of subjects with ALT events in the Prava group, and sought to correlate this potential beneficial effect with the reduction in lipids that was also seen.

 

Results:  

Changes in mean ALT values were analyzed in 38 and 43 CHCV pts randomized to Prava and placebo respectively and correlated with the percentage change in total cholesterol (TC), LDL, and triglycerides (TGs) over the 36 wks of the trial. Using a 2 sample t-test, we observed a modest reduction in ALT values at nearly all time points over the 36 wk trial among the Prava recipients (ranging up to a 6% decrease from baseline), while ALT values on placebo tended to increase (as much as 33%). At several time points, the comparative difference in the change in ALT approached statistical significance (e.g. p=0.0637). These reductions in ALT on Prava appeared to correlate with the highly statistically significant reductions in lipids seen on Prava compared to placebo (p <0.0001).

 

Summary:

Pravastatin Efficacy in HCV

·        Pravastatin 80 mg daily statistically lowered total cholesterol and LDL-C at week 12 compared with placebo in subjects with chronic hepatitis C

·        Mean lipid values at other time points (weeks 4,8,24 and 36) also showed similar statistical significance favoring pravastatin.

Pravastatin Safety in HCV

·        ALT events were not statistically different between pravastatin and placebo recipients at any time during the 36 week study in patients underlying compensated chronic hepatitis C.

·        However, the incidence of ALT events was lower at all time points compared to placebo in hepatitis C patients (possible treatment effect?).

·        No difference in serious AEs or discontinuations due to AEs between the groups.

 

Conclusions:  

This subgroup analysis of our larger prospective, randomized, double-blind, placeo-controlled trial demonstrates the efficacy and safety of high-dose Prava in hypercholesterolemic subjects with CHCV. In addition to the significant reduction of TC, LDL and TGs, there was also a trend toward a significant numerical reduction in ALT values on Prava compared to placebo for the patients with CHCV. While the mechanism for the ALT reduction is uncertain, the results warrant further study relative to the interrelationship between hepatitis C and serum lipids.

 


1132. Risk Factors for Relapse in Genotype 3 High Viral Load Patients with Hepatitis C In the WIN-R Trial. 

R. Brown; I. Jacobson; A. Nezam; B. Freilich; M. Pauly; F. Regenstein; S. Flamm; P. Kwo; S. Becker; L. Griffel; C. Brass.

 

Aim:  

Patients with G2/3 hepatitis C virus (HCV) have high SVR rates to PEG-IFN alfa-2b + RBV and require shorter duration of treatment. G3 patients with a baseline high viral load (HVL) have lower response rates and higher relapse rates than G2 patients (Table), but predictors of relapse are not known. The purpose of this analysis was to determine the predictors of relapse to PEG-IFN alfa-2b + RBV in patients with HCV G3.

 

Methods:

In the WIN-R trial, which was a multicenter, randomized, open-label, investigator-initiated trial in 225 US sites, treatment-naïve HCV patients with compensated liver disease were randomized to receive PEG-IFN alfa-2b 1.5μg/kg/wk + fixed dosing (FD; 800mg/d) or weight-based dosing (WBD; 800 mg/d, <65kg, 1000mg/d, 65–85kg; 1200mg/d, >85–105kg; 1400mg/d, >105–125kg) of RBV for 24 or 48 wks. HCV RNA was assessed by PCR (Taqman/SPRI, LLQ 29IU/mL) at wks 0, 24, 48 and 72. The primary endpoint was SVR (HCV RNA negative at wk 72) in patients ≥65kg. Multivariate regression analyses were used to analyze predictors of relapse.

 

Results:  

G2/3 patients (n=1829) were enrolled and randomized to WBD (24 wks n=317, 48 wks n=602) or FD (24 wks n=322, 48 weeks n=588). With WBD and 24 wks of therapy, SVR rates were higher and relapse lower with G2 than G3 patients (SVR: 72% vs 63% and relapse: 5% vs 11%). Relapse rates were highest among G3 HVL patients treated for 24 weeks (16%). Univariate analyses revealed G3 (vs G2), viral load (high vs low) and duration of treatment (24 vs 48 weeks) were associated with higher relapse rates in Caucasians. African Americans had a lower relapse rate and no difference between G2 and G3. Multivariate analyses controlling for the above, race, and steatosis revealed only G2 vs G3 as a predictor of relapse. G3 HVL patients had similar relapse rates with WBD/48 wks of therapy than with FD/24 wks therapy.

Summary:

·        Overall, G2 patients experienced higher SVR rate3s and lower relapse rates than G3 patients.

·        Relapse rates were highest among G3 high viral load patients treated fro 24 weeks.

·        Multivariate analysis revealed G3 patients (versus) G2 patients as the only predictor of relapse.

·        Similar to G2 patients, when controlling for viral load, G3 patients do no appear to benefit from longer duration of therapy or WBD ribavirin.

Conclusions:

 

 

SVR, %

Relapse Rate, %

 

WBD vs FD

WBD vs FD

G2 patients

72/68 vs 71/62

6/3 vs 6/66

LVL (24/48 wks)

71/66 vs 73/66

6/5 vs 6/7

HVL (24/48 wks)

69/59 vs 74/58

7/2 vs 8/6

G3 patients

63/54 vs 57/49

10/11 vs 10/13

LVL (24/48 wks)

65/56 vs 61/51

6/9 vs 7/16

HVL (24/48 wks)

70/52 vs 53/55

18/16 vs 14/7

 


1133. IMPACT OF TARIBAVIRIN AND RIBAVIRIN EXPOSURE ON EFFICACY AND ANEMIA RATES WHEN COMBINED WITH PEGYLATED INTERFERON ALFA-2b IN THE TREATMENT OF CHRONIC HCV. 

I. Jacobson ; P. Pockros; Y. Benhamou; R. Esteban-Mur; Y. Lurie; R. Flisiak; N. Afdhal; Y. Kim; Y. Xu; B. Murphy.

 

Introduction:

A recent Phase 3 trial examining the efficacy and safety of fixed-dose taribavirin when combined with PEG IFN alfa 2b vs weight base dosed ribavirin (RBV) plus PEG IFN alfa 2b revealed that the taribavirin treated pts exhibited a significantly lower anemia rate (5% vs 24%;p<0.001) but failed to meet the non-inferiority criterion when analyzed for efficacy (38% vs 52%). Taribavirin is a liver-targeting pro-drug of RBV and is converted to the active moiety by the enzyme adenosine deaminase. Since the current standard of care in the management of HCV is to weight base drug exposure of RBV, this post-hoc analysis looked at the impact on efficacy and the development of anemia (hemoglobin<10g/dL) of drug exposure based on weight(mg/kg).

 

Methods:  

This Phase 3 study randomized 970 pts in a 2:1 ratio into a fixed dose taribavirin arm vs a weight base dosed RBV arm, stratifying pts on the basis of genotype, baseline viral load, and weight. In thi